Article
New mutations in and around the L2 disordered loop of the RecA protein modulate recombination and/or coprotease activity.
Journal of bacteriology - 1 Oct 1992
Larminat F, Cazaux C, Germanier M, Defais M
Abstract excerpt
The RecA protein plays a key role in Escherichia coli recombination and DNA repair. We have created new recA mutants with mutations in the vicinity of the recA430 mutation (Gly-204----Ser) which is known to affect RecA coprotease activity. Mutants carrying recA659 or recA611, located 3 and 7 amino acids downstream of residue 204, respectively, lose all RecA activities, while the mutant carrying recA616, which is...
Topics
- Alleles
- Bacterial Proteins
- Endopeptidases
- Escherichia coli
- Genes, Dominant
- Mutagenesis, Site-Directed
- Rec A Recombinases
- Recombination, Genetic
- SOS Response, Genetics
- Serine Endopeptidases
- Structure-Activity Relationship