Inducibility of the SOS response in a recA730 or recA441 strain is restored by transformation with a new recA allele.

doi:10.1007/BF00277070

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Article

Inducibility of the SOS response in a recA730 or recA441 strain is restored by transformation with a new recA allele.

Molecular & general genetics : MGG - 1 Aug 1993

Cazaux C, Mazard A M, Defais M

Abstract excerpt

Escherichia coli RecA protein plays an essential role in both genetic recombination and SOS repair; in vitro RecA needs to bind ATP to promote both activities. Residue 264 is involved in this interaction; we have therefore created two new recA alleles, recA664 (Tyr264-->Glu) and recA665 (Tyr264--...

Topics

Alleles
Cloning, Molecular
Escherichia coli
Genes, Bacterial
Genetic Complementation Test
Mutagenesis, Site-Directed
Point Mutation
Rec A Recombinases
Recombination, Genetic
SOS Response, Genetics
Transformation, Bacterial

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