Heterogeneity of messenger RNA that encodes the rat insulin receptor is limited to the domain of exon 11. Analysis by RNA heteroduplex mapping, amplification of cDNA, and in vitro translation.

Abstract excerpt

Structural isoforms of the insulin receptor that occur in various tissues have been postulated to be involved in certain actions of insulin in target cells. To determine whether these insulin-receptor subtypes are caused by alterations in the receptor primary structure, we used RNA heteroduplex mapping and amplification of cDNA to detect variation in the coding region of insulin-receptor mRNA from 5 rat tissues....

Topics

• Animals
• Antisense Elements (Genetics)
• Base Sequence
• DNA
• Exons
• Genetic Variation
• Male
• Molecular Sequence Data
• Molecular Weight
• Nucleic Acid Heteroduplexes
• Oligodeoxyribonucleotides