Sequencing of eleven introns in genomic DNA encoding rat glucagon receptor and multiple alternative splicing of its mRNA.

doi:10.1016/0014-5793(94)00875-2

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Article

Sequencing of eleven introns in genomic DNA encoding rat glucagon receptor and multiple alternative splicing of its mRNA.

FEBS letters - 5 Sept 1994

Maget B, Tastenoy M, Svoboda M

Abstract excerpt

We used the PCR (polymerase chain reaction) to amplify fragments of glucagon receptor DNA from genomic DNA. Sequencing of the subcloned fragments demonstrated that genomic DNA encoding the glucagon receptor spans over 12 exons interrupted by 11 introns. The introns were located mainly at the 5' e...

Topics

Alternative Splicing
Animals
Cloning, Molecular
Genetic Variation
Genome
Introns
Models, Genetic
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger
Rats
Receptors, Glucagon
Sequence Analysis, DNA

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