Article
Characterization of monoclonal antibodies recognizing three distinct, phosphorylated carbohydrate epitopes in the lipopolysaccharide of the deep rough mutant I-69 Rd-/b+ of Haemophilus influenzae.
Molecular microbiology - 1 Feb 1997
Rozalski A, Brade L, Kosma P, Moxon R, Kusumoto S, Brade H
Abstract excerpt
Monoclonal antibodies against the lipopolysaccharide (LPS) of the deep rough mutant I-69 Rd-/b+ of Haemophilus influenzae were obtained after immunization of mice with sheep erythrocytes which had been coated with de-O-acylated LPS. Characterization of antibodies was performed by enzyme immuno assay (EIA) using LPS or neoglycoconjugates containing partial structures of LPS as solid-phase antigens and by...
Topics
- Animals
- Antibodies, Monoclonal
- Binding, Competitive
- Blotting, Western
- Epitopes
- Fluorescent Antibody Technique, Direct
- Glycoproteins
- Haemophilus influenzae
- Lipopolysaccharides
- Mice