Direct demonstration of the infiltration of murine central nervous system by Pgp-1/CD44high CD45RB(low) CD4+ T cells that induce experimental allergic encephalomyelitis.

Abstract excerpt

In experimental allergic encephalomyelitis (EAE), autoimmune T cells infiltrate the central nervous system (CNS) and initiate demyelinating pathology. We have used flow cytometry to directly analyse the migration to the CNS of MBP-reactive CD4+ T cells labelled with a lipophilic fluorescent dye (PKH2), in SJL/J mice with passively transferred EAE. Labelled cells constituted about 45% of the CNS CD4+ population at...

Topics

• Animals
• Antigens, CD
• CD4 Antigens
• Cell Movement
• Cell Separation
• Central Nervous System
• Encephalomyelitis, Autoimmune, Experimental
• Female
• Fluorescent Dyes
• Histocompatibility Antigens