Article
Amplification of substoichiometric recombinant mitochondrial DNA sequences in a nuclear, male sterile mutant regenerated from protoplast culture in Nicotiana sylvestris.
Molecular & general genetics : MGG - 1 May 1992
Vitart V, De Paepe R, Mathieu C, Chétrit P, Vedel F
Abstract excerpt
A Nicotiana sylvestris plant regenerated from protoplast culture was found to be mutated in both the mitochondrial (mt) and nuclear genomes. The novel mt DNA organization, called U, is due to the amplification of recombinant substoichiometric DNA sequences that preexist in the parent line. The recombination event involves two 404 bp repeats, which hybridize to a 2.1 kb transcript. Although the sequence of both...
Topics
- Amino Acid Sequence
- Base Sequence
- Chloroplasts
- Cloning, Molecular
- Cosmids
- DNA, Mitochondrial
- DNA, Recombinant
- Gene Amplification
- Homozygote
- Molecular Sequence Data