Article

Somatic cybridization between Nicotiana tabacum and N. repanda based on a single inactivation procedure of nuclear donor parental protoplasts.

2005-02-01

Abstract excerpt

In the present study, mesophyll protoplasts isolated from N. tabacum var. K326 were inactivated by Rhodamine-6G (5 μg/ml, 15 min), and induced by PEG (6000) to fuse with those from N. repanda. All 31 plants regenerated from fusion-derived protoplast cultures showed uniform characteristics identical to K326 except flower morphology and male sterility. Morphological, cytological, biochemical investig...

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Literature Corpus work: 01f5cd71-a79c-5862-b9c9-b6a003bebc74
DOI: 10.1016/j.plantsci.2004.05.026

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