Article
Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, Cyanidioschyzon merolae 10D.
Plant & cell physiology - 1 Jun 2004
Minoda Ayumi, Sakagami Rei, Yagisawa Fumi, Kuroiwa Tsuneyoshi, Tanaka Kan
Abstract excerpt
Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plates. Using these...
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Culture Techniques
- Cell Nucleus
- Cells, Cultured
- Culture Media
- DNA, Plant
- Molecular Sequence Data
- Multienzyme Complexes
- Mutation
- Orotate Phosphoribosyltransferase
- Orotic Acid
- Orotidine-5'-Phosphate Decarboxylase