Article
Several nuclear genes control both male sterility and mitochondrial protein synthesis in Nicotiana sylvestris protoclones.
Molecular & general genetics : MGG - 1 Jul 1990
De Paepe R, Chétrit P, Vitart V, Ambard-Bretteville F, Prat D, Vedel F
Abstract excerpt
Male sterile plants appeared in the progeny of three fertile plants obtained after one cycle of protoplast culture from a fertile botanical line and two androgenetic lines of Nicotiana sylvestris. These plants showed the same foliar and floral abnormalities as the cytoplasmic male sterile (cms) mitochondrial variants obtained after two cycles of culture. We show that male sterility in these plants is controlled...
Topics
- Cloning, Molecular
- Crosses, Genetic
- Culture Techniques
- DNA, Mitochondrial
- Electrophoresis, Polyacrylamide Gel
- Genetic Complementation Test
- Genetic Variation
- Mitochondria
- Plant Proteins
- Plants, Toxic
