Article
Cytoplasmic male sterility is associated with large deletions in the mitochondrial DNA of two Nicotiana sylvestris protoclones.
Current genetics - 1 Feb 1992
Chetrit P, Rios R, De Paepe R, Vitart V, Gutierres S, Vedel F
Abstract excerpt
Two cytoplasmic male-sterile plants (CMSI and CMSII) were obtained by protoplast culture in Nicotiana sylvestris. Both plants showed large deletions (up to 50 kb) in their mitochondrial DNA. Restriction maps of the reorganized regions suggested that the deletions occurred via two homologous recom...
Topics
- Blotting, Southern
- Cells, Cultured
- Chromosome Deletion
- Cloning, Molecular
- Cosmids
- Cytoplasm
- DNA, Mitochondrial
- Models, Genetic
- Mutation
- Plants, Toxic
- Protoplasts
- Recombination, Genetic