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Article

Two monoclonal antibodies recognizing different epitopes on rat cytochrome IIB1 react with human IIE1.

Molecular pharmacology - 1 Jan 1992

Wrighton S A, Vandenbranden M, Becker G W, Black S D, Thomas P E

Abstract excerpt

To identify human cytochromes P450 (P450) in the CYP2B subfamily, 14 human liver microsomal samples were screened by immunoblots developed with monoclonal antibodies that recognized seven distinct epitopes on rat IIB1. Two of these antibodies recognized a protein in all of the samples. This protein was termed P450BE. Using video-imaging densitometry, the levels of P450BE were determined and compared with levels...

Topics

Adolescent
Adult
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Cytochrome P-450 CYP2B1
Cytochrome P-450 CYP2E1
Cytochrome P-450 Enzyme System
Densitometry
Electrophoresis, Polyacrylamide Gel

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Epitopes

Female

Humans

Immunoblotting

Immunohistochemistry

Isoenzymes

Male

Mice

Source

Identifiers

PMID: 1370708

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1370708-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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