Article
An inhibitory monoclonal antibody binds in close proximity to a determinant for substrate binding in cytochrome P450IIC5.
The Journal of biological chemistry - 5 Apr 1991
Kronbach T, Johnson E F
Abstract excerpt
We used the expression of chimeric proteins and point mutants to identify amino acids of the hepatic progesterone 21-hydroxylase P450IIC5 which are part of an epitope recognized by an inhibitory monoclonal antibody and which affect substrate binding. Three amino acids of P450IIC5 at positions 113, 115, and 118 were introduced into P450IIC4, which is 95% identical to P450IIC5. The resultant chimeric protein...
Topics
- Alanine
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Blotting, Western
- Catalysis
- Chimera
- Cytochrome P-450 Enzyme System
- Cytochrome P450 Family 2
- Epitopes
- Kinetics