Selection and characterization of alpha-amylase-overproducing recombinant Escherichia coli containing the bacterial hemoglobin gene.

doi:10.1007/BF00174475

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Article

Selection and characterization of alpha-amylase-overproducing recombinant Escherichia coli containing the bacterial hemoglobin gene.

Applied microbiology and biotechnology - 1 Nov 1992

Liu S C, Ogretmen B, Chuang Y Y, Stark B C

Abstract excerpt

We previously reported that the presence of the bacterial (Vitreoscilla) hemoglobin gene enhances alpha-amylase production in recombinant Escherichia coli strain MK79. Using the growth of MK79 on starch as a selective method we have produced a mutant strain (BSC9) that produces up to four times a...

Topics

Bacillus subtilis
Cloning, Molecular
Escherichia coli
Genes, Bacterial
Gram-Negative Chemolithotrophic Bacteria
Hemoglobins
Mutation
Species Specificity
alpha-Amylases

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