Chromosomal transformation in Saccharomyces cerevisiae with DNA isolated by pulse field gel electrophoresis.

Article

Chromosomal transformation in Saccharomyces cerevisiae with DNA isolated by pulse field gel electrophoresis.

Agricultural and biological chemistry - 1 Jun 1990

Goto K, Motoyoshi T, Tamura G, Obata T, Hara S

Abstract excerpt

We isolated and purified yeast chromosome DNA molecules using pulse field gel electrophoresis (PFG). The isolated DNA had nearly the same size as the native chromosomal DNA on PFG. We could directly transform Saccharomyces cerevisiae yeasts with it, and obtain transformants that were selected by...

Topics

DNA, Fungal
Electrophoresis, Agar Gel
Genotype
Karyotyping
Phenotype
Saccharomyces cerevisiae
Transformation, Genetic

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