Article
Chromosomal transformation in Saccharomyces cerevisiae with DNA isolated by pulse field gel electrophoresis.
Agricultural and biological chemistry - 1 Jun 1990
Goto K, Motoyoshi T, Tamura G, Obata T, Hara S
Abstract excerpt
We isolated and purified yeast chromosome DNA molecules using pulse field gel electrophoresis (PFG). The isolated DNA had nearly the same size as the native chromosomal DNA on PFG. We could directly transform Saccharomyces cerevisiae yeasts with it, and obtain transformants that were selected by complementation of several markers. They had new chromosome DNA bands observed on PFG. The new chromosome was very...
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