Article
Pulse Field Gel Electrophoresis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016
Sharma-Kuinkel Batu K, Rude Thomas H, Fowler Vance G
Abstract excerpt
Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction. PFGE is a variation of agarose gel electrophoresis that permits analysis of bacterial DNA fragments over an order of magnitude...
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