Article
Structural analyses of sugar chains from ricin A-chain variant.
Agricultural and biological chemistry - 1 Jan 1990
Kimura Y, Kusuoku H, Tada M, Takagi S, Funatsu G
Abstract excerpt
Two glycopeptides were isolated from a tryptic digest of ricin A-chain variant by gel filtration and reversed-phase HPLC. Their amino acid compositions and sequences showed that Asn-10 and Asn-236 are glycosylated. The sugar chains were liberated from these glycopeptides by hydrazynolysis, N-acetylated, and pyridylaminated. Component analysis and comparison of elution positions of the purified pyridylamino (PA-)...
Topics
- Carbohydrate Conformation
- Carbohydrate Sequence
- Chromatography, Gel
- Chromatography, High Pressure Liquid
- Chromatography, Ion Exchange
- Electrophoresis, Polyacrylamide Gel
- Genetic Variation
- Glycopeptides
- Molecular Sequence Data
- Peptide Fragments