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Article

A CHO strain producing high-level human IL-6 with the 3' deletion construct.

Journal of biotechnology - 1 Feb 1992

Tonouchi N, Koyama N, Miwa K

Abstract excerpt

A Chinese Hamster Ovary (CHO) strain producing high-level human interleukin 6 (IL-6) was obtained, by introduction of the IL-6 cDNA from which the 3' AU-rich region was deleted. The IL-6 mRNA of this strain was stable. The productivity was more than 15 times higher than the previously obtained clone, which has intact IL-6 cDNA in the same expression vector. This strain produced IL-6 at a high level of 30...

Topics

Animals
Blotting, Northern
Blotting, Southern
CHO Cells
Cloning, Molecular
Cricetinae
Gene Amplification
Humans
Interleukin-6
Kinetics
Mutation
RNA, Messenger

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1367984
DOI: 10.1016/0168-1656(92)90146-z

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1367984-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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