Article
Enzyme engineering for nonaqueous solvents. II. Additive effects of mutations on the stability and activity of subtilisin E in polar organic media.
Biotechnology progress - 1 Jan 2000
Chen K Q, Robinson A C, Van Dam M E, Martinez P, Economou C, Arnold F H
Abstract excerpt
Single amino acid substitutions increase the activity and stability of subtilisin E in mixtures of organic solvents and water, and the effects of these mutations are additive. A variant of subtilisin E that exhibits higher activity in mixtures of dimethylformamide (DMF) and water (Q103R) was created by random mutagenesis combined with screening for improved activity (K. Chen and F. H. Arnold, in preparation)....
Topics
- Chemical Phenomena
- Chemistry, Physical
- Dimethylformamide
- Enzyme Activation
- Enzyme Stability
- Mutation
- Protein Engineering
- Solvents
- Subtilisins
- Water