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Article

Purification of cloned proaerolysin released by a low protease mutant of Aeromonas salmonicida.

Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 1990

Buckley J T

Abstract excerpt

The precursor to the hole-forming toxin aerolysin has been purified in high yield from culture supernatants of a mutant of Aeromonas salmonicida containing the cloned structural gene. The mutant strain was generated by Tn5 mutagenesis. It released little or no protease or other extracellular prot...

Topics

Aeromonas
Bacterial Toxins
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Genes, Bacterial
Hemolysin Proteins
Humans
Mutation
Peptide Hydrolases
Pore Forming Cytotoxic Proteins
Protein Precursors
Recombinant Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 2190617
DOI: 10.1139/o90-029

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2190617-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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