Article
Purification of cloned proaerolysin released by a low protease mutant of Aeromonas salmonicida.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 1990
Buckley J T
Abstract excerpt
The precursor to the hole-forming toxin aerolysin has been purified in high yield from culture supernatants of a mutant of Aeromonas salmonicida containing the cloned structural gene. The mutant strain was generated by Tn5 mutagenesis. It released little or no protease or other extracellular proteins, including phospholipase, suggesting that it is a regulatory mutant. The absence of protease allowed the isolation...
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