Article
Allelic diversity at BoLA DRB3 locus and association with predisposition to clinical mastitis in indicus and crossbred cattle.
Animal biotechnology - 1 Nov 2023
Loat Shubham, Kumari Namita, Saini Shallu, M S Dige, Kumar Anurag, Dhilor Nitika, Dang Ajay Kumar, Lathwal S S, Sodhi Monika, Kataria Ranjit S
Abstract excerpt
Bovine lymphocyte antigen (BoLA) DRB3 locus in healthy and mastitis affected cattle has been genotyped by a polymerase chain reaction and restriction fragment length polymorphisms (PCR-RLFP) using RsaI restriction enzyme, followed by sequencing. In 130 farm animals, 25 BoLA DRB3 alleles have been detected by PCR-RFLP. Three distinct allelic patterns significantly associated with mastitis in Karan Fries crossbred...
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