Article
Characterization of mutant forms of the quinoprotein methanol dehydrogenase lacking an essential calcium ion.
The Biochemical journal - 1 Nov 1992
Richardson I W, Anthony C
Abstract excerpt
Methanol dehydrogenase (MDH) from Methylobacterium extorquens, Methylophilus methylotrophus, Paracoccus denitrificans and Hyphomicrobium X all contained a single atom of Ca2+ per alpha 2 beta 2 tetramer. The role of Ca2+ was investigated using the MDH from Methylobacterium extorquens. This was shown to be similar to the MDH from Hyphomicrobium X in having 2 mol of prosthetic group (pyrroloquinoline quinine; PQQ)...
Topics
- Alcohol Oxidoreductases
- Calcium
- Coenzymes
- Gram-Negative Aerobic Bacteria
- Kinetics
- Mutation
- PQQ Cofactor
- Quinolones
- Spectrum Analysis