Article
Site-directed mutagenesis and X-ray crystallography of the PQQ-containing quinoprotein methanol dehydrogenase and its electron acceptor, cytochrome c(L).
Biochemistry - 21 Aug 2001
Afolabi P R, Mohammed F, Amaratunga K, Majekodunmi O, Dales S L, Gill R, Thompson D, Cooper J B, Wood S P, Goodwin P M, Anthony C
Abstract excerpt
Two proteins specifically involved in methanol oxidation in the methylotrophic bacterium Methylobacterium extorquens have been modified by site-directed mutagenesis. Mutation of the proposed active site base (Asp303) to glutamate in methanol dehydrogenase (MDH) gave an active enzyme (D303E-MDH) with a greatly reduced affinity for substrate and with a lower activation energy. Results of kinetic and deuterium...
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