Article
Development of an insertional expression vector system for Methylobacterium extorquens AM1 and generation of null mutants lacking mtdA and/or fch.
Microbiology (Reading, England) - 1 Jan 2004
Marx Christopher J, Lidstrom Mary E
Abstract excerpt
Over the past few years, the genetic 'toolkit' available for use with Methylobacterium extorquens AM1 has expanded significantly. Here a further advance is presented and demonstrated, an insertional expression system that allows expression of genes from a stable, unmarked chromosomal locus. This system has been used to better understand the role of the tetrahydrofolate (H4F) pathway in methylotrophy. Previously,...
Topics
- Formates
- Genes, Bacterial
- Genetic Vectors
- Methenyltetrahydrofolate Cyclohydrolase
- Methylobacterium
- Methylobacterium extorquens
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Oxidoreductases Acting on CH-NH Group Donors
- Phenotype
- Plasmids