Article
Isolation, phenotypic characterization, and complementation analysis of mutants of Methylobacterium extorquens AM1 unable to synthesize pyrroloquinoline quinone and sequences of pqqD, pqqG, and pqqC.
Journal of bacteriology - 1 Mar 1994
Morris C J, Biville F, Turlin E, Lee E, Ellermann K, Fan W H, Ramamoorthi R, Springer A L, Lidstrom M E
Abstract excerpt
Aerobic gram-negative methylotrophs oxidize methanol to formaldehyde by using a methanol dehydrogenase that has pyrroloquinoline quinone (PQQ) as a prosthetic group. Seventy-two mutants which are unable to grow on methanol unless the growth medium is supplemented with PQQ have been isolated in th...
Topics
- Amino Acid Sequence
- Base Sequence
- Genes, Bacterial
- Genetic Complementation Test
- Gram-Negative Aerobic Bacteria
- Molecular Sequence Data
- Mutation
- PQQ Cofactor
- Phenotype
- Quinolones
- Sequence Homology, Amino Acid