Article
Genotype-specific in vitro amplification of sequences of the wild type 3 polioviruses from Mexico and Guatemala.
Virus research - 1 Aug 1992
Yang C F, De L, Yang S J, Ruiz Gómez J, Cruz J R, Holloway B P, Pallansch M A, Kew O M
Abstract excerpt
The extensive nucleotide sequence heterogeneity among independent genotypes of wild polioviruses permits the systematic design of genotype-specific molecular reagents. We have prepared two sets of polymerase chain reaction (PCR) primer pairs specific for the genotype of wild poliovirus type 3 rec...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- DNA, Single-Stranded
- Genotype
- Guatemala
- Mexico
- Molecular Sequence Data
- Poliovirus
- Poliovirus Vaccine, Oral
- Polymerase Chain Reaction
- RNA, Viral
- Sensitivity and Specificity