Article
Deletion and transposon mutagenesis and sequence analysis of the pRO1600 OriR region found in the broad-host-range plasmids of the pQF series.
Plasmid - 1 May 1994
Jansons I, Touchie G, Sharp R, Almquist K, Farinha M A, Lam J S, Kropinski A M
Abstract excerpt
The nucleotide sequence of the replicative origin (OriR) region of the small cryptic broad-host-range plasmid, pRO1600, which forms the basis of a number of useful cloning vectors has been determined. In addition it has been subjected to Tn5 mutagenesis, deletion analysis, and subcloning in order...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Calorimetry
- Cloning, Molecular
- DNA Helicases
- DNA Replication
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Genetic Vectors
- Genotype
- Molecular Sequence Data
- Mutagenesis, Insertional
- Oligodeoxyribonucleotides
- Plasmids
- Pseudomonas aeruginosa