Article
Saturation mutagenesis in Escherichia coli of a cloned Xanthomonas campestris DNA fragment with the lux transposon Tn4431 using the delivery plasmid pDS1, thermosensitive in replication.
Applied microbiology and biotechnology - 1 Nov 1993
Steinmann D, Wiggerich H G, Klauke B, Schramm U, Pühler A, Priefer U B
Abstract excerpt
A system allowing transposon mutagenesis of cloned DNA fragments in Escherichia coli with Tn4431, which carries the promotorless luciferase (lux) operon of Vibrio fischeri, has been developed. The transposon delivery plasmid, pDS1, based on an IncF replicon, is thermosensitive in replication and...
Topics
- Cloning, Molecular
- DNA Replication
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli
- Hot Temperature
- Luminescent Measurements
- Mutagenesis, Insertional
- Phenotype
- Plasmids
- Xanthomonas campestris