Article
Yeast mitochondrial DNA mutators with deficient proofreading exonucleolytic activity.
The EMBO journal - 1 Jul 1992
Foury F, Vanderstraeten S
Abstract excerpt
The MIP1 gene which encodes yeast mitochondrial DNA polymerase possesses in its N-terminal region the three motifs (Exo1, Exo2 and Exo3) which characterize the 3'-5' exonucleolytic domain of many DNA polymerases. By site directed mutagenesis we have substituted alanine or glycine residues for con...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA Polymerase I
- DNA Repair
- DNA Replication
- DNA, Fungal
- DNA, Mitochondrial
- Electrophoresis, Polyacrylamide Gel
- Exonucleases
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype