Site-directed mutagenesis in rat liver 6-phosphofructo-2-kinase. Mutation at the fructose 6-phosphate binding site affects phosphate activation.

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Article

Site-directed mutagenesis in rat liver 6-phosphofructo-2-kinase. Mutation at the fructose 6-phosphate binding site affects phosphate activation.

The Journal of biological chemistry - 5 Mar 1992

Li L, Lin K, Kurland I J, Correia J J, Pilkis S J

Abstract excerpt

To identify those residues involved in fructose 6-phosphate binding to the kinase domain of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase site-directed mutations were engineered at Lys194, Arg195, Arg230, and Arg238. The mutant enzymes were purified to homogeneity by anion exchan...

Topics

Animals
Arginine
Base Sequence
Binding Sites
Chromatography, Gel
Circular Dichroism
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Fructosephosphates
Kinetics
Liver
Lysine

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutagenesis, Site-Directed

Mutation

Phosphates

Phosphofructokinase-2

Phosphotransferases

Source

Open in PubMed

Identifiers

PMID: 1311308

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1311308-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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