Article
Site-directed mutagenesis of Lys-174, Asp-179 and Asp-191 in the 2-kinase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
The Biochemical journal - 1 Feb 1997
Bertrand L, Deprez J, Vertommen D, Di Pietro A, Hue L, Rider M H
Abstract excerpt
In a structural model of the 2-kinase domain of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase based on the analogy with adenylate kinase, Lys-174, Asp-179 and Asp-191 residues are located in the putative active site. Asp-179 and Asp-191 are conserved in all known 6-...
Topics
- Adenosine Triphosphate
- Animals
- Circular Dichroism
- Cloning, Molecular
- Enzyme Stability
- Escherichia coli
- Fructose-Bisphosphatase
- Gene Expression
- Guanidine
- Guanidines
- Hydrogen-Ion Concentration
- Kinetics
- Liver
- Models, Chemical
- Mutagenesis, Site-Directed
- Mutation
- Phosphofructokinase-1
- Phosphofructokinase-2