Thermodynamics of denaturation of mutants of barnase with disulfide crosslinks.

doi:10.1006/jmbi.1997.0928

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Article

Thermodynamics of denaturation of mutants of barnase with disulfide crosslinks.

Journal of molecular biology - 25 Apr 1997

Johnson C M, Oliveberg M, Clarke J, Fersht A R

Abstract excerpt

We have measured the effects of disulfide crosslinks on the thermodynamics of denaturation of three mutants of barnase that contain cystine and the corresponding single and double cysteine mutants. At first sight, the data are consistent with the hypothesis that disulfide crosslinks stabilise pro...

Topics

Bacterial Proteins
Cysteine
Disulfides
Hot Temperature
Hydrogen-Ion Concentration
Mutation
Protein Denaturation
Protein Folding
Ribonucleases
Thermodynamics

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