Article
Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs.
The Plant journal : for cell and molecular biology - 1 May 1992
Minet M, Dufour M E, Lacroute F
Abstract excerpt
An Arabidopsis thaliana cDNA bank has been constituted in a Saccharomyces cerevisiae expression vector based on the phosphoglycerate kinase (PGK) promoter and terminator. This bank was used to complement eight S. cerevisiae auxotrophic markers. All of them were corrected. These results confirm th...
Topics
- Amino Acid Sequence
- Arabidopsis
- Base Sequence
- Cloning, Molecular
- DNA
- Dihydroorotate Dehydrogenase
- Gene Library
- Genetic Complementation Test
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Oxidoreductases
- Oxidoreductases Acting on CH-CH Group Donors
- Plants
- Saccharomyces cerevisiae