Article
Method to express and purify nm23-H2 protein from baculovirus-infected cells.
BioTechniques - 1 Aug 2003
Garzia L, André A, Amoresano A, D'Angelo A, Martusciello R, Cirulli C, Tsurumi T, Marino G, Zollo M
Abstract excerpt
High-throughput protein expression and purification are major bottlenecks in the postgenomic and proteomic era. We show here an automated method to express and purify nm23-H2, a nucleoside diphosphate kinase (NDPK), in a 96-well format, by the use of a robotic workstation, from insect Spodoptera frugiperda (Sf9) baculovirus-infected cells using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads. The automated...
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