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Article

Single nucleotide polymorphism discrimination assisted by improved base stacking hybridization using oligonucleotide microarrays.

BioTechniques - 1 Aug 2003

Wang D, Gao H, Zhang R, Ma X, Zhou Y, Cheng J

Abstract excerpt

Efficiencies of mismatch discrimination using size-varied capture probes were examined at various hybridization temperatures. The probes were 17, 15, 13, 11, 9, and 7 nucleotides long and contained single-base mismatches at their 3' ends. The optimal signal intensity and efficiency of base stacking hybridization on mismatch discrimination were observed for capture probes with a melting temperature (Tm) value of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 12951771
DOI: 10.2144/03352st02

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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