Article
Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase.
Nucleic acids research - 15 Aug 2003
Davidson John F, Fox Richard, Harris Dawn D, Lyons-Abbott Sally, Loeb Lawrence A
Abstract excerpt
Insertion of the T3 DNA polymerase thioredoxin binding domain (TBD) into the distantly related thermostable Taq DNA polymerase at an analogous position in the thumb domain, converts the Taq DNA polymerase from a low processive to a highly processive enzyme. Processivity is dependent on the presence of thioredoxin. The enhancement in processivity is 20-50-fold when compared with the wild-type Taq DNA polymerase or...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.