Article
[Recombinant virus assay: a rapid assay for assessment of drug susceptibility of human immunodeficiency virus type 1 isolates].
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae - 1 Oct 2002
Qin Shu-lin, Arendt Victor, Hemmer Robert, Schneider Francois, Wang Ai-xia, Sheng Rui-yuan
Abstract excerpt
OBJECTIVE: To establish a rapid assay for assessment of drug susceptibility of human immunodeficiency virus type 1 isolates (Recombinant virus assay). METHODS: This procedure allows the generation of viable virus with SI phenotype by homologous recombination of a RT-PCR-derived pool of reverse transcriptase (RT) coding sequences into an RT-deleted, noninfectious proviral clone, pHIV delta RTBstE II. Then the drug...
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