Article
Regulated expression of the Escherichia coli dam gene.
Journal of bacteriology - 1 Aug 2003
Calmann Melissa A, Marinus M G
Abstract excerpt
Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.
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