Article
Bacteriophage P1 Ban protein is a hexameric DNA helicase that interacts with and substitutes for Escherichia coli DnaB.
Nucleic acids research - 15 Jul 2003
Lemonnier Marc, Ziegelin Günter, Reick Tobias, Muñoz Gómez Ana, Díaz-Orejas Ramón, Lanka Erich
Abstract excerpt
Since the ban gene of bacteriophage P1 suppresses a number of conditionally lethal dnaB mutations in Escherichia coli, it was assumed that Ban protein is a DNA helicase (DnaB analogue) that can substitute for DnaB in the host replication machinery. We isolated and sequenced the ban gene, purified the product, and analysed the function of Ban protein in vitro and in vivo. Ban hydrolyses ATP, unwinds DNA and forms...
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