Article
Identification of functional regulatory regions of the connexin32 gene promoter.
Biochimica et biophysica acta - 9 Jul 2003
Field Joanne M L, Tate Laura A, Chipman J Kevin, Minchin Stephen D
Abstract excerpt
Connexin32 (Cx32) is the predominant gap junction protein expressed in adult rat hepatocytes. This study investigated transcriptional regulation of the rat Cx32 gene in MH(1)C(1) rat hepatoma cells using transient expression assays in conjunction with promoter mutagenesis and 5' nested deletion analysis. Site-directed mutagenesis of the -736 and -187 hepatocyte nuclear factor-1 (HNF-1) sites, the -196 and -116...
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