Article
Trimeric mutant bacteriorhodopsin, D85N, shows a monophasic CD spectrum.
FEBS letters - 25 Oct 1993
Kataoka M, Mihara K, Kamikubo H, Needleman R, Lanyi J K, Tokunaga F
Abstract excerpt
The structure of mutant bacteriorhodopsin (bR), D85N, was examined by CD and X-ray diffraction at pH 7. The absorption maximum of D85N at pH 7 is located at 605 nm, which is similar to the acid-blue form of wild-type bR. D85N shows a monophasic CD band, the maximum of which is at 575 nm, although the crystalline arrangement and the trimeric structure is maintained. The acid-blue form of wild-type bR shows a...
Topics
- Bacteriorhodopsins
- Circular Dichroism
- Hydrogen-Ion Concentration
- Mutation
- X-Ray Diffraction