Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

In vitro DNA-binding properties of VirB, the Shigella flexneri virulence regulatory protein.

FEBS letters - 19 Jun 2003

McKenna Sorcha, Beloin Christophe, Dorman Charles J

Abstract excerpt

The DNA-binding activity of the Shigella flexneri VirB transcription factor was studied in vitro. The protein was found to bind non-specifically to DNA, but showed preferential binding to VirB-dependent promoter sequences. DNA binding was contingent on the presence of an intact helix-turn-helix motif. While high molecular mass protein-DNA complexes were formed in both specific and non-specific interactions with...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Gene Expression Regulation, Bacterial
Share to Helix-Turn-Helix Motifs

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to In Vitro Techniques

Share to Leucine Zippers

Share to Molecular Weight

Share to Oligopeptides

Share to Shigella flexneri

Share to Virulence Factors

Source

Identifiers

PMID: 12804772
DOI: 10.1016/s0014-5793(03)00524-6

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

In vitro DNA-binding properties of VirB, the Shigella flexneri virulence regulatory protein.PMID 12804772Read ↗

The role of the VirB ligand CTP in the molecular mechanism of transcriptional anti-silencing in Shigella flexneri.Semantic proximity Read ↗

The role of the VirB ligand CTP in the molecular mechanism of transcriptional anti-silencing in <i>Shigella flexneri</i>Semantic proximity Read ↗

The virulence regulator VirB from <i>Shigella flexneri</i> uses a CTP-dependent switch mechanism to activate gene expressionSemantic proximity Read ↗

The Multifaceted Activity of the VirF Regulatory Protein in the Shigella LifestyleSemantic proximity Read ↗

Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity.Semantic proximity Read ↗

Deregulation of temperature-dependent transcription of the invasion regulatory gene, virB, in Shigella by rho mutation.Semantic proximity Read ↗

The regulatory VirG protein specifically binds to a cis-acting regulatory sequence involved in transcriptional activation of Agrobacterium tumefaciens virulence genes.Semantic proximity Read ↗

Analysis of abrB mutations, mutant proteins, and why abrB does not utilize a perfect consensus in the -35 region of its sigma A promoter.Semantic proximity Read ↗

A single amino acid change in the transmembrane domain of the VirB8 protein affects dimerization, interaction with VirB10 and Brucella suis virulence.Semantic proximity Read ↗

Mutational analysis of the nucleoid-associated protein HBsu of Bacillus subtilis.Semantic proximity Read ↗

Characterization of a single-stranded DNA-binding protein from Klebsiella pneumoniae: mutation at either Arg73 or Ser76 causes a less cooperative complex on DNA.Semantic proximity Read ↗

Structural analysis of the DNA-binding domain of the Erwinia amylovora RcsB protein and its interaction with the RcsAB box.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.