Article
In vitro detection of dissimilar amounts of hepatitis C virus (HCV) subtype-specific RNA genomes in mixes prepared from sera of persons infected with a single HCV genotype.
Journal of clinical microbiology - 1 Jun 2003
Quarleri Jorge F, Bussy María V, Mathet Verónica L, Ruiz Vanesa, Iácono Rubén, Lu Ling, Robertson Betty H, Oubiña José R
Abstract excerpt
The level of in vitro detection of viral genomes in mixes with two different hepatitis C virus (HCV) subtypes was investigated by artificially mixing previously measured subtype-specific HCV RNA genomes. The RNAs in these mixtures were reverse transcribed and then PCR amplified by using two sets of primers corresponding to the 5' untranslated region and digested with endonucleases to analyze the restriction...
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