Article
Growth of cultured porcine retinal pigment epithelial cells.
Acta ophthalmologica Scandinavica - 1 Apr 2003
Wiencke Anne Katrine, Kiilgaard Jens Folke, Nicolini Jair, Bundgaard Magnus, Röpke Carsten, La Cour Morten
Abstract excerpt
PURPOSE: To establish and characterize cultures of porcine retinal pigment epithelial (pRPE) cells in order to produce confluent monolayers of cells for transplantation. METHODS: Primary pRPE cell cultures were established. Cell morphology was assessed by phase contrast and electron microscopy. Growth was determined by the crystal violet dye uptake assay. DNA synthesis and content were measured by incorporation...
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