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Article

Enzyme I of the phosphotransferase system: induced-fit protonation of the reaction transition state by Cys-502.

Biochemistry - 29 Apr 2003

García-Alles Luis Fernando, Alfonso Ignacio, Erni Bernhard

Abstract excerpt

Enzyme I (EI), the first component of the phosphoenolpyruvate (PEP):sugar phosphotransferase system (PTS), consists of an N-terminal domain with the phosphorylation site (His-189) and a C-terminal domain with the PEP binding site. Here we use C3-substituted PEP analogues as substrates and inhibitors and the EI(C502A) mutant to characterize structure-activity relationships of the PEP binding site. EI(C502A) is 10...

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PMID: 12705838
DOI: 10.1021/bi034007f

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PTS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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