Article
In vivo retroviral gene transfer by direct intrafemoral injection results in correction of the SCID phenotype in Jak3 knock-out animals.
Blood - 1 Aug 2003
McCauslin Christine S, Wine John, Cheng Linzhao, Klarmann Kim D, Candotti Fabio, Clausen Peter A, Spence Sally E, Keller Jonathan R
Abstract excerpt
Efficient retroviral gene transfer to pluripotential hematopoietic stem cells (PHSCs) requires ex vivo culture in multiple hematopoietic growth factors (HGFs) to promote cell division. While treatment of PHSCs with HGF can render stem cells viable targets for retroviral infection, HGFs can promote differentiation, loss of self-renewal potential, and affect the homing/engraftment capacity of PHSCs. To avoid the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.