Article
Nucleofection is an efficient nonviral transfection technique for human bone marrow-derived mesenchymal stem cells.
Stem cells (Dayton, Ohio) - 1 Feb 2006
Aluigi Michela, Fogli Miriam, Curti Antonio, Isidori Alessandro, Gruppioni Elisa, Chiodoni Claudia, Colombo Mario P, Versura Piera, D'Errico-Grigioni Antonia, Ferri Elisa, Baccarani Michele, Lemoli Roberto M
Abstract excerpt
Viral-based techniques are the most efficient systems to deliver DNA into stem cells because they show high gene transduction and transgene expression in many cellular models. However, the use of viral vectors has several disadvantages mainly involving safety risks. Conversely, nonviral methods are rather inefficient for most primary cells. The Nucleofector technology, a new nonviral electroporation-based gene...
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