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Article

Design of molecular beacons for AmpliDet RNA assay--characterization of binding stability and probe specificity.

Analytical biochemistry - 15 Apr 2003

Szemes Marianna, Schoen Cor D

Abstract excerpt

AmpliDet RNA is a real-time diagnostic method, the specificity of which is defined mainly by the molecular beacon (MB). MBs can be characterized according to the stability of their stem-and-loop structures and that of the probe-target duplex via the free energies accompanying their formation. By the application of thermodynamic models, we propose a prediction method for these deltaG(0) parameters, which was...

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PMID: 12689829
DOI: 10.1016/s0003-2697(02)00692-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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