Article
Proteomic analysis of eIF-5A in lung adenocarcinomas.
Proteomics - 1 Apr 2003
Chen Guoan, Gharib Tarek G, Thomas Dafydd G, Huang Chiang-Ching, Misek David E, Kuick Rork D, Giordano Thomas J, Iannettoni Mark D, Orringer Mark B, Hanash Samir M, Beer David G
Abstract excerpt
We examined the eIF-5A protein expression in 93 lung adenocarcinomas and 10 uninvolved lung samples using quantitative two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) analysis with identification by mass spectrometry and 2-D Western blots. The same tissue samples were examined for the eIF-5A mRNA expression using oligonucleotide microarrays, and the cellular localization of the eIF-5A protein was...
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