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Article

Reconstruction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB.

Applied and environmental microbiology - 1 Apr 2003

Nagata Yuji, Prokop Zbynek, Marvanová Sona, Sýkorová Jana, Monincová Marta, Tsuda Masataka, Damborský Jirí

Abstract excerpt

The homology model of protein Rv2579 from Mycobacterium tuberculosis H37Rv was compared with the crystal structure of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26, and this analysis revealed that 6 of 19 amino acid residues which form an active site and entrance tunnel are different in LinB and Rv2579. To characterize the effect of replacement of these six amino acid residues, mutations were...

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Identifiers

PMID: 12676719
DOI: 10.1128/AEM.69.4.2349-2355.2003
PMCID: PMC154798

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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